Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Activation of coagulation FXI promotes endothelial inflammation and amplifies platelet activation in a nonhuman primate model of hyperlipidemia

doi: 10.1016/j.rpth.2023.102276

Figure Lengend Snippet: Levels of activated FXI were elevated in a model of hyperlipidemia. PPP was isolated from NHPs on a standard chow diet ( n = 6, lean) or NHPs on a high-fat diet ( n = 8, obese). Clotting times were measured by incubating PPP with an aPTT reagent followed by CaCl 2 to initiate clot formation (A) or by stimulating PPP with Dade Innovin Reagent to measure PT. Activated coagulation protease species, FXIa–AT and T-AT, were measured using a custom ELISA (C, D). Statistical analyses were conducted using a Mann–Whitney test or an unpaired t -test. Statistical significance is indicated by an asterisk for P ≤ .05. Data are shown as mean ± SEM. aPTT, activated partial thrombospondin time; AT, antithrombin; ELISA, enzyme-linked immunosorbent assay; F, factor; PPP, platelet-poor plasma; PT, prothrombin time; NHP, nonhuman primate.

Article Snippet: Plasma samples were analyzed for C-reactive protein using an enzyme-linked immunosorbent assay kit (ELISA, ALPCO) following the manufacturer’s instructions.

Techniques: Isolation, Coagulation, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Activation of coagulation FXI promotes endothelial inflammation and amplifies platelet activation in a nonhuman primate model of hyperlipidemia

doi: 10.1016/j.rpth.2023.102276

Figure Lengend Snippet: FXI inhibition prolonged aPTT clotting times in a model of hyperlipidemia. PPP was isolated from NHPs on a high-fat diet ( n = 5) treated with a FXI function–blocking antibody. Clotting times were measured by incubating PPP with an aPTT reagent followed by CaCl 2 to initiate clot formation (A) or by stimulating PPP with Dade Innovin Reagent to measure PT (B). Activated coagulation protease species, FXIa–AT and T–AT, were measured using a custom ELISA (C, D). Statistical analyses were conducted using a Friedman test with a Dunn’s post-hoc test or repeated measures one-way ANOVA with a Dunnett’s post-hoc test. Statistical significance is indicated by an asterisk for P < .05. Data are shown as mean ± SEM. aPTT, activated partial thrombospondin time; AT, antithrombin; ELISA, enzyme-linked immunosorbent assay; F, factor; NHP, nonhuman primate; PPP, platelet-poor plasma; PT, prothrombin time.

Article Snippet: Plasma samples were analyzed for C-reactive protein using an enzyme-linked immunosorbent assay kit (ELISA, ALPCO) following the manufacturer’s instructions.

Techniques: Inhibition, Coagulation, Isolation, Blocking Assay, Enzyme-linked Immunosorbent Assay